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Transcriptomic features of Cr GPDH expression in mammalian cells To further understand the effect of Cr GPDH expression on metabolism, we performed a bulk RNA sequencing (RNA-seq) analysis of HeLa cells with Cr GPDH expression ( Figure 5a-b )

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However, at this level of analysis, it is difficult to know exactly how the opposing expression patterns of these particular enzymes between the long-lived glp-1(-) and fem-3(-) mutants and the short-lived mog-3(-) mutant result in the intriguing difference in sphingosine levels among the three mutants