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Detection of intracellular ROS and mitochondrial ROS Senescent ATDC5 cells were cultured with CuS and B2M-CuS NPs (50 g mL 1 ) for 24 h, and intracellular ROS was detected via a 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) method as we previously reported [36, 37]

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Compounds such as NCX4040 deplete intracellular GSH and elevate lipid peroxidation markers, and their effects are significantly enhanced when combined with ferroptosis inducers such as erastin or RSL3 (35)