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At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore

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Elevated glutamine metabolism makes cancer cells more susceptible to glutamine deficiency caused by glutaminase inhibitors, which rapidly induces cell death [122]