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Cigars are most often used as a means of celebration for graduations, birthdays and promotions, they said
In the second step, 7 l cDNA from step-1 was amplified using N gene forward primer (5-CACATTGGCACCCGCAATC-3), N gene reverse primer (5-GAGGAACGAGAAGAGGCTTG-3) and probe (5-FAM-ACTTCCTCAAGGAACAACATTGCCA-QSY-3) using Taqman mastermix (Thermo Fisher)
We also used these regressions to examine whether a quadratic trend might be better than a linear trend but found no evidence of this (the quadratic coefficients were negligible and not statistically significant)
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