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First, preliminary dose-response curves were established by treating LGECs with CGA (0-2000 nM), MK-2206 (010 M), and SC79 (0-100 ng/ml) for 24 h to determine non-toxic concentrations

Free Radic Res 54(1112):841847 Huang H et al (2023) Genetically engineered mesenchymal stem cells as a nitric oxide reservoir for acute kidney injury therapy

Following a 30-min incubation in the dark at room temperature, 5 10 4 cells were counted and cell cycle analysis (ratio of G0/G1 and S + G2/M phases) was performed with a flow cytometer (Cytoflex

It is essential to identify the role of Gln metabolism at various stages of cancer and evaluate its overall efficacy to achieve optimal clinical outcomes