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Wnt signalling in gastrointestinal epithelial stem cells
After blocking, the membranes were incubated overnight at 4 C with the following primary antibodies: PFKFB3 (1:2000, Proteintech, Wuhan, China, 13763-1-AP), NLRP3 (1:500, Novus, Littleton, CO, USA, NBP2-12446), ASC (1:1000, Cell Signaling Technology, Danvers, MA, USA, 67824s), Caspase-1 p20 (1:1000, Proteintech, Wuhan, China, 22915-1-AP), collagen I (1:2000, Proteintech, Wuhan, China, 14695-1-AP), -SMA (1:4000, Proteintech, Wuhan, China, 14395-1-AP), -actin (1:5000, Proteintech, Wuhan, China, 20536-1-AP), Pan-Kla (1:1000, PTM Biolabs, Hangzhou, China, PTM-1401RM), H4K5la (1:1000, PTM Biolabs, Hangzhou, China, PTM-1407RM), H4K12la (1:1000, PTM Biolabs, Hangzhou, China, PTM-1411RM), H3K18la (1:1000, PTM Biolabs, Hangzhou, China, PTM-1406RM), H4 (1:2000, PTM Biolabs, Hangzhou, China, PTM-1004), H3 (1:2000, PTM Biolabs, Hangzhou, China, PTM-1001), and p300 (1:1000, Cell Signaling Technology, Danvers, MA, USA, 67621)
Both freezing and excessive heat can damage the medication
