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Regulation of androgen synthesis affects follicular development and ovarian function through multiple pathways: Androgen-promoting pathways: IRS-PI3K/cAMP/PKA, MAPK/ERK (via FGF13/LH/CYP17), NF-B (via TNF- activation/CYP17 up-regulation), and miR-130b-3p (targeting MAPK1/PI3K) all promote androgen production

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A subset of samples was also prepared in degassed water under oxygen-free conditions in an anaerobic glovebox (Coylab) or under aerobic conditions following bubbling under N 2 gas for 1 h with continuous stirring in a round bottom flask