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Ecotoxicology 14:589596 Le Faucheur S, Behra R, Sigg L (2005) Phytochelatin induction, cadmium accumulation, and algal sensitivity to free cadmium ion in Scenedesmus vacuolatus

The membrane was washed three times in Tris-buffered saline with Tween 20 (TBS-T) for 10 min, and treated with HRP-conjugated anti-rabbit or anti-mouse IgG (GE Healthcare) for 1 h

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Endogenous APD is considered an autoimmune condition because someone who has APD reacts to progesterone when their immune system mistakenly identifies progesteronea hormone naturally produced by the body, with peak levels around day 21 of a 28-day menstrual cycle as a harmful substance