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Symptoms as the disease progresses Besides an ulcer, the following symptoms may occur as floor of the mouth cancer progresses: Pain in the oral cavity that may sometimes spread to the nearest ear Bleeding (without any prior injury) at the affected area Bad breath Difficulty in eating, speaking and swallowing Swollen cervical lymph nodes Additionally, symptoms of generally feeling unwell, e.g

After static incubation at 30 C for 24 h, the bacteria were collected and the total bacteria RNA were extracted with EasyPure RNA Purification Kit (TransGen Biotech, Beijing, China) following the manufacturers protocol

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