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Sholl analysis, performed with a plugin in ImageJ software (NIH, version 1.53c.), automatically drew concentric circles from the center of the DAPI signal outward to the ends of the astrocyte processes, analyzing the number of intercepts and the ramification index

Similarly, preclinical research involving animals must comply with Institutional Animal Care and Use Committee (IACUC) directives and adhere to Animal Welfare Act (AWA) standards

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