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Cell culture SH-SY5Y cells were grown in Dulbeccos Modified Eagles Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and cultured at 37 C under humidified 5% CO 2 atmosphere

In clinical settings, IV administration allows glutathione to bypass digestion and enter circulation directly, offering more predictable absorption. According to Patel, IVs can be effective in the short-term, but can create sharp spikes in blood levels that rise quickly and fade just as fast. After years of research, Patel developed a patented sub-nano topical delivery system designed to transport reduced glutathione across the skin barrier and into circulation, which can be found in his Auro Wellness products