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To record mPTP opening, the suspension of organelles was used for 24 h mPTP opening was investigated by spectrophotometric registration of the swelling of mitochondria isolated from the rat heart ( 2 PO 4 3, sodium succinate5, pH 7.4, and a decrease in the optical density in mitochondria suspension was recorded at = 520 nm for 15 min of the mitochondria swelling