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It is a structured refeed that targets specific hormonal mechanisms, particularly leptin resensitization

19 This precise targeting mechanism ensures that OSK functions only in senescent cells that highly express Cdkn2a , restoring these senescent cells to a more youthful state and prolonging the lifespan of aging mice

1999;274(1):516-521.[DOI] 17

After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader