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Synthesis of cDNA was performed with 1 g of total RNA from each sample using Quantiscript Reverse Transcriptase, Quantiscript RT-buffer and RT Primenr-mix of QuantiTect Reverse Transcription kit (Qiagen GmbH, Hilden, Germany) according to the manufacturers protocol

However, if you are deficient in vitamin E, supplementation may be a good idea (37)

Statistical analysis Statistical analysis was performed by parametric one-way ANOVA with post hoc Newman-Keuls test and non-parametric Kruskal-Wallis and subsequent Mann-Whitney U -test to compare groups

Besides, 100 mg SU6668 and 5 mg indocyanine green (ICG) were dissolved in 10 mL mixed solution of DMSO and ethanol, following the same method as above, resulting in the precipitation of NanoSU6668 (ICG)