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For subcutaneous injection, pinch the skin to create a fold, insert the needle at a 45-90 degree angle, and inject slowly

Handling : Use sterile techniques when handling Bacteriostatic Water to prevent contamination

Specifically, to assess basal analgesia, (i) the first assessment (time "0") was preceded by the application of morphine or saline 30 min before (time "-30 min"), then by the application of the gastric pentadecapeptide BPC 157 or naloxone or saline 20 min before (time "-20 min"), and finally, by the application of the saline 10 min before (time "-10 min")