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Traditionally, this activation was attributed to inhibition of mitochondrial complex I, which raises the AMP:ATP ratio (LaMoia and Shulman, 2021

Controlled human trials exist, but they're limited in size, focused mostly on topical cosmetic use, and usually last only a few weeks or months. That means a lot of the exciting claims, especially around gene expression, systemic repair, and anti-aging effects, haven't been confirmed in large-scale human studies

Inhibition of poly(ADP-ribose) polymerase: reduction of ischemic injury and attenuation of N-methyl-D-aspartate-induced neurotransmitter dysregulation

Microdosing may lead to unpredictable side effects and interactions with other medications