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The functional activity of FVII from supernatants of iPSC-HO was assessed by two methods: a) western blotting after activation with tissue factor (TF, STA Neoplastin) and CaCl 2 and incubation with antithrombin (AT), purified from human plasma, (Grifols, Spain) and unfractionated heparin (Hospira) to measure the ability of FVII to become active and form a complex with AT
To do this, carefully add the diluent to the vial and gently swirl it until the powder is fully dissolved
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Neuropil A dense network of interwoven axons, dendrites, unmyelinated fibres and synaptic connections in the grey matter of the central nervous system, crucial for information processing, neuronal communication and cognition
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